A cover slip was placed over the semen droplet, and the percent of motile spermatozoa was microscopically estimated at ×400 magnification according to Bearden and Fuquay . Rabbits were ranked by restricted randomization procedures that approximately equalized the initial bwts among the different groups. More specifically, it is not approved by the Food and Drug Administration for use in humans for any reason.

Products intentionally adulterated with high amounts of ‘classic’ anabolic steroids, including boldione , metandienone, stanozolol, dehydrochloromethyltestosterone, oxandrolone, etc., have been found in the nutritional supplement market. Moreover, these anabolic steroids may not be declared on the product labels. USADA offers guidance on on how to reduce the risk of using contaminated or spiked supplements. Boldenone is an anabolic androgenic steroid and synthetic derivative of testosterone that was originally developed for veterinary use but has since become one of the more common performance-enhancing drugs that athletes test positive for in sport. The undecylenate ester of the anabolic steroid boldenone, developed for veterinary use, mostly for treatment of horses. Teale P, Houghton E. The development of a gas chromatographic/mass spectrometric screening procedure to detect the administration of anabolic steroids to the horse.


Incidence and severity of lesions in BOL-treated groups are summarized in . The National Institute of Standards and Technology uses its best efforts to deliver a high quality copy of the Database and to verify that the data contained therein have been selected on the basis of sound scientific judgment. However, NIST makes no warranties boldenone to that effect, and NIST shall not be liable for any damage that may result from errors or omissions in the Database. We are focused on providing unbeaten customer service, whilst delivering the highest quality products all around the world. Studies about percentage of extraction of some hormones using ATPS in aqueous samples.

A review on structural elucidation of metabolites of environmental steroid hormones via liquid chromatography–mass spectrometry

In a study to propose the fragmentation pathway of protonated estrone , Bourcier et al. The proposal of fragmentation pathways of a specific compound depends not only on knowledge of fragmentation reactions but also other valuable approaches. Particularly, the use of HRAM-MS enables the calculation of the molecular formulae of both precursor and product ions. Afterwards, the nitrogen rule can be applied to and the ring double-bond equivalent value can be calculated from the molecular formula. A positive mono-charged ion has a RDB value ending in +0.5 and a negative mono-charge ion has a RDB value ending in −0.5. Besides, stable-isotope labeling, multistage fragmentation and certain software are also very much helpful.

Histopathological alterations in the testes and epididymis were marked in the group C rabbits. These results indicate that administration of BOL exerts a significant harmful effect on the reproductive functions of male rabbits. There have been relatively few studies which have investigated the detrimental effects of BOL administration on male function. The detection of drugs in human hair samples has been performed by laboratories around the world for many years and the matrix is popular in disciplines, such as workplace drug testing. To date, however, hair has not become a routinely utilised matrix in sports drug detection. The analysis of hair samples offers several potential advantages to doping control laboratories, not least of which are the greatly extended detection window and the ease of sample collection and storage.

Fed pelleted commercial feed (Ibex Co., Cairo, Egypt) and water was supplied without restriction. Rabbits in all groups received humane care in compliance with the animal care guidelines of the National Institute of Health, and the local ethical committee approved this study. In addition to being illegally sold and used for human performance enhancement, boldenone is used illegally in racehorses to enhance performance, despite being banned for use in horses competing in Olympic and Paralympic events. On an international and agricultural level, boldenone is also largely illegal for use as a growth promotor in farm animals, and most countries have surveillance programs that test for boldenone in animals raised for food production.

Quality Control of Boldenone undecylenate

Laboratory analysis showed a detectable clenbuterol serum concentration. Analysis of an unopened ampule contained Boldenone undecylenate, clenbuterol, and vitamin E. This case illustrates a novel exposure that caused beta-agonist toxicity and was treated successfully with rapid-onset beta blocker. Clark AS, Henderson LP. Behavioral and physiological responses to anabolic-androgenic steroids. No detectable changes were noticed in both treated groups apart from some moderate tubular dilatation . There are many known cases of doping in sports with boldenone undecylenate by professional athletes.

This study was conducted on forty male rats which are divided into four groups . We also noted a significant increase in the levels of cardiac malondialdehyde, H2O2 generation in heart tissues, mRNA expression of NOX2 and NOX4, and immunoreactivity to proliferating cell nuclear antigen . Posttreated rats with Boldenone and GSPE ameliorated cardiac toxicity via inhibition of NOX and a reduction in alteration of the expression of NOX2, NOX4, and PCNA induced by Boldenone.

It was observed that phase II metabolites could also be generated even without the addition of cofactors. To the authors’ knowledge, this is the first report of the successful use of homogenized horse liver for the generation of phase II metabolites. It also demonstrates the ease with which both phase I and phase II metabolites can now be generated in vitro simply by using homogenized liver without the need for ultracentrifuges or tedious preparation steps. Meanwhile, Lalonde et al. reported the use of 2D-LC to purify eight underivatized steroids, including testosterone, dehydroepiandrosterone and metabolites, prior to isotope analysis. They demonstrated improved purity with reduced analysis time compared to a standard 1D HPLC procedure. Boldenone (androsta-1,4-dien-17β-ol-3-one) is an anabolic steroid synthesized by dehydrogenation of testosterone.


No significant abnormalities in the sperm morphology were found in all treated groups compared with the control group . Group A rabbits served as control group and received 0.25 ml sesame oil/kg bwt. Group B received 4.4 mg/kg bwt boldenone undecylenate 5% oily solution (Equi-gan®; Lab Tornel, Co., Mexico). All groups were injected intramuscularly twice weekly for 2 months. The doses of BOL were calculated according to Paget and Barnes . Thirty white New Zealand mature male rabbits, 9–9.5 months of age, were housed in metal cages.

This study was conducted to evaluate the adverse effects of the anabolic steroid, boldenone undecylenate on reproductive functions of male rabbits. Thirty white New Zealand mature male rabbits were divided into three groups . Group B rabbits received 4.4 mg/kg body weight BOL 5% oily solution. Rabbits were injected intramuscularly twice weekly for two months.

Boldenone Undecylenate is a synthetic steroid which has a similar effect as the natural steroid testosterone; it is frequently used in veterinary medicine, though it is also used in humans. If you are in the process of experiment, it’s necessary to make the dilution ratios of the samples. Normally, it’s can get a better solubility within lower of Concentrations. Boldenone is an analbolic steroid mostly used in veterinary practice, and has also been used by professional athletes for performance and muscle enhancement. Investigated the CID spectra of a series of standard steroids and correlated the diagnostic fragmentation pattern with their structural feature, which holds promise guiding the structural elucidation of their metabolites.

Testes and epididymis weights were decreased significantly in the BOL-treated groups. BOL caused significant reduction in serum testosterone level, seminal volume, sperm motility, and sperm count. No abnormalities were detected in the sperm morphology of the BOL-treated groups.


Along with being prohibited in sport, athletes should be aware of the many health and safety issues surrounding boldenone. Clenbuterol is a beta-agonist that has been abused by fitness-oriented individuals for muscle growth and weight loss. We report a case of a 46-year-old man who presented tachycardic, hypokalemic, and hyperglycemic after injecting testosterone obtained from Brazil. He developed refractory hypotension and was started on an esmolol infusion for suspected beta-agonist toxicity.

Metabolites appearing in urine in horses are 17α-hydroxy-epimers as glucuronic acid conjugates and unchanged drug with 17β-hydroxy compounds as sulphate conjugates. And methylboldenone are anabolic steroids often illegally used to boost animal growth during the breeding of animals for human consumption, thereby causing potential health risks to consumers. The abuse of AAS can lead to serious and irreversible organ damage (Maravelias et al. 2005). In 2018, there were 87 cases involving positive tests for boldenone, which represents 6 percent of all positives for anabolic agents globally in WADA-accredited laboratories. Squires EL, Todter GE, Berndtson WE, Pickett BW. Effect of anabolic steroids on reproductive function of young stallion. Maher JM, Squires EL, Voss JL. Shideler RK. Effect of anabolic steroids on reproductive function of young mares.

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